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Whose View of Life? – Embryos, Cloning and Stem Cells whose view of life – embryos cloning and stem cells
Whose View of Life? – Embryos, Cloning and Stem Cells
1827 RUR
Derivation of embryonic stem cells in buffalo whose view of life – embryos cloning and stem cells
This monograph will have practical use to those who are interested to establish embryonic stem cells laboratory. Embryonic stem cells are one of the emerging areas in reproductive biotechnologies in buffalo, which could have enormous agricultural and biomedical applications. Besides its immense potential in humans, the stem cell technology has proved to be a boon for farm animals since promising results have been obtained in the fields of gene targeting, cloning, transgenesis, chimera formation and differentiation into germ cells (sperm and oocytes). This book mainly deals with the isolation, culture, characterization and differentiation of embryonic stem cells generated from in vitro fertilized embryos in buffalo and grown on different feeder cells. It will also give the overview of preparation of feeder cells, cryopreservation, in vitro fertilization and parthenogenetic activation.
5790 RUR
Embryonic Stem Cell-like Cells in Goat (Capra hircus) whose view of life – embryos cloning and stem cells
The objective of the present experiment was production of goat embryos either by parthenogenetic activation or by in vitro fertilization and derivation of embryonic stem (ES) cell-like cells. Firstly, the effects of different activation methods, culture systems and culture media on in vitro development of zona-free and with-zona parthenogenetic embryos were investigated. Secondly, effect of cysteamine supplementation in the in vitro maturation (IVM) or culture (IVC) media, or a combination of both in terms of blastocyst production by in vitro fertilization was investigated. Finally, different methods of isolation of ES cell-like cells from in vitro produced embryos were compared and effects of Leukaemia Inhibitory Factor (LIF) and different types of feeder layers on growth of ES cell-like cells were investigated. The undifferentiated ES cell-like cells showed stem cell like morphological features, normal karyotype, expressed stem cell specific surface and intracellular markers and maintained undifferentiated state for long periods in culture. Mechanical isolation of ICMs from hatched blastocysts was found to be the best method to establish goat ES cell-like cells.
4839 RUR
Microchimeric Fetal Stem cells in Pregnancy whose view of life – embryos cloning and stem cells
Stem cells are one of the keys to life,they potentially nourish,heal and protect us. Stem cells are truly remarkable. They bridge the gap between our origin and what we become. They supply the cells that construct our adult bodies and, as we age, replenish damaged and diseased tissues. A true stem cell is a primitive cell capable of unlimited cell cycles with the capacity to cease being a stem cell and become one of a wide variety of specialized cells that can no longer divide Fetal stem cells cross into maternal blood during pregnancy and thus represent a potential non- invasive source of foetal genetic material for prenatal diagnosis.
4839 RUR
Heide Schatten Cell and Molecular Biology and Imaging of Stem Cells whose view of life – embryos cloning and stem cells
Cell and Molecular Biology and Imaging of Stem Cells features original and review articles written by experts who have made significant contributions to stem cell biology and imaging. Chapters cover a broad spectrum of aspects of the field, including Stem cells from the Amniotic Fluid and Placenta, Biomaterials as Artificial Niches for Pluripotent Stem Cell Engineering, Low-Intensity Ultrasound in Stem Cells and Tissue Engineering, Mammalian Neo-Oogenesis from Ovarian Stem Cells, Oct4-EGFP Transgenic Pigs as a New Tool for Visualization of Pluripotent and Reprogrammed Cells, Regulation of Adult Intestinal Stem Cells, Stem Cell Therapy for Veterinary Orthopedic Lesions, Sex Steroid Combinations in Regenerative Medicine for Brain and Heart Diseases, Hair Follicle Stem Cells, The Potential of Using Induced Pluripotent Stem Cells in Skin Diseases, Mitochondrial Differentiation in Early Embryo Cells and Pluripotent Stem Cells, and The Role of Centrosomes in Cancer Stem Cell Functions. Cell and Molecular Biology and Imaging of Stem Cells addresses a wide variety of cell and molecular topics in unprecedented detail, and is a must-read for graduate students and academic and industry professionals in the expanding field of stem cell biology. Reviews new imaging tools and markers for cell and molecular imaging in stem cell biology. Covers novel aspects of stem cell imaging in reproductive biology and stem cell niches Includes chapters on the developing area of centrosome biology as it applies to embryonic and adult stem differentiation
9883.84 RUR
Stem cells whose view of life – embryos cloning and stem cells

Stem cells

4839 RUR
Stem cells whose view of life – embryos cloning and stem cells
Cell is the structural and functional unit of life. A living cell is a true representative of life with its own organisation and specialised functions. Scientists have been interested in cell biology since the advent of microscopes in 18th century. Stem cells are defined as clonogenic, unspecialised cells capable of both self renewal for long periods and multilineage differentiation contributing to regenerate specific tissues. They can theoretically divide without limit to replenish other cells as long as the person is still alive. Research on stem cells and their application to treat various diseases is still at a preliminary stage. However, results from animal models are very promising and many researchers believe that it is only a matter of time before the same results can be achieved with human stem cell.
4839 RUR
Ethical Perspective of Stem Cell Therapy for Cancer whose view of life – embryos cloning and stem cells
The use of stem cells for research, whether from children, adults, aborted fetuses, or embryos, must be done under conditions of the most rigorous integrity for several reasons. The use of adult stem cell requires the same conditions as those required in the case of tissue donation, based on respect for the integrity of the human body and the free and informed consent of the donor. The use of foetal tissues to derive stem cells requires, besides informed consent, that no abortion is induced for the purpose of obtaining the tissues and that the termination timing and the way it is carried out are not influenced by this retrieval. This book is a try to usher light on path of ethical requirements for carrying out stem cell research in cancer therapy.
4839 RUR
Dental pulp as a new source of stem cells whose view of life – embryos cloning and stem cells
Stem cells are one of the wonders of biology. Some of the reasons for using these cells include the possibility to save their proliferation during cell cycle and their ability to differentiate into various types of mature cells, whereas normal cell can only give rise to its kind. Stem cells are divided into two with respect to their source viz embryonic stem cells and adult stem cells. Considering the limitations of working with embryonic stem cells, there is a growing interest among researchers to focus their studies on adult stem cells. Adult stem cells are found in many organs and tissues, but the important factor is the number of cells in each tissue is very limited. Accordingly, the present study investigates the cultivation of adult stem cells in dental pulp as a potential rich source of these cells. Dental pulp stem cells (DPSC) do not require general anesthesia during extraction as compared to bone marrow stem cells. They also have a very high proliferation rate when cultured and their properties are most closely related to the embryonic stem cells.
3549 RUR
Vinagolu Rajasekhar K. Cancer Stem Cells whose view of life – embryos cloning and stem cells
Cancer Stem Cells covers a wide range of topics in cancer stem cell biology, including the functional characteristics of cancer stem cells and how they're generated, where they are localized, the means by which cancer stem cells can be targeted, and how cancer stem cells can be reprogrammed back to normal tissue stem cells. Each chapter begins with a brief historical note and concept summary, followed by a description of the latest basic or clinical advance associated with the topic. Cancer Stem Cells builds systematically from coverage of the basic research stage to an advanced research level, from clinical relevance to therapeutic potential, and will be a valuable resource for professionals in the fields of cancer research and stem cell biology.
12243.97 RUR
Phenotypes of Stem cells from Dental Pulp & Periodontal Ligament whose view of life – embryos cloning and stem cells
It has been established that human dental pulp and periodontal ligament contain a population of mesenchymal stem cells (MSCs). However, the phenotypic analysis in terms of putative stem cell markers expressed by these stem cell populations is incomplete. It is relevant to understand whether stem cells derived from closely related tissues are programmed differently. This book therefore, provides an insight into the phenotypes of dental pulp stem cells (DPSCs) and periodontal ligament stem cells (PDLSCs). Both the stem cells express embryonic stem (ES) cell markers. Differences were observed in expression of stemness-related markers. DPSCs displayed increased percentages of SSEA4, CD13 and CD166 and decreased CD9 expression compared to PDLSCs. Both stem cells express common MSC markers, different levels of expression suggests there might be more than one stem cell population existing within these tissues which differ in their embryonic status, and DPSCs are a more primitive stem cell population in comparison to PDLSCs. This analysis should shed some light into the nature of stem cells from dental tissues and help researchers utilize these stem cells for regenerative medicine.
3549 RUR
Mechanical Stimulation of Dental Pulp Stem Cells whose view of life – embryos cloning and stem cells
This book presents a study that focuses on the concepts of functional tissue engineering (FTE) and stem cell technology in an attempt to apply such technologies in the dental field. In this study, we focused on the effect of mechanical compression on stimulating the odontogenic differentiation of dental pulp stem cells. The pulp cells were isolated, their differentiation potentials to the angiogenic and neurogenic lineages were tested, and were stimulated mechanically in a custom built bioreactor to differentiate into odontoblasts. The results were promising, showing the ability of mechanical stimulation to induce the odontogenic differentiation of the isolated pulp stem cells. This book should be useful to researchers in the fields of tissue regeneration and stem cells technology or anyone who is interested in learning the concepts of stem cells isolation and differentiation.
5490 RUR
Thyagarajan Bhaskar Primary and Stem Cells. Gene Transfer Technologies and Applications whose view of life – embryos cloning and stem cells
This book describes basic cell engineering methods, emphasizing stem cell applications, and use of the genetically modified stem cells in cell therapy and drug discovery. Together, the chapters introduce and offer insights on new techniques for engineering of stem cells and the delivery of transgenes into stem cells via various viral and non-viral systems. The book offers a guide to the types of manipulations currently available to create genetically engineered stem cells that suit any investigator's purpose, whether it's basic science investigation, creation of disease models and screens, or cells for therapeutic applications.
8100.99 RUR